Biological Information
Background Information:
The ADRA1A Gq cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses ADRA1A , a naturally Gq coupled GPCR. Upon activation of ADRA1A , the resulting calcium activity can be fluorescently assayed using the Calcium NoWash PLUS Detection Kit.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Adrenoceptor
Sub Family:
Class A
Coupling:
Gq
Protein Name:
alpha1A
Protein Aliases:
Alpha-1A adrenoceptor|Alpha-1C adrenergic receptor|Alpha-adrenergic receptor 1c
Accession Number:
NM_000680
UniProt Number:
P35348
Gene Name:
ADRA1A
Gene ID:
148
Gene Aliases:
ADRA1L1|ADRA1C
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Chemiluminescence
Additional Information
Brand:
Eurofins DiscoverX®