PathHunter™ EFC whole-cell-format for protein:protein interaction
and protein trafficking

With PathHunter™ assays, developing assays for protein trafficking and protein:protein interaction is easy and can
be studied in whole cell context using standard 96-, 384- or 1535-well protocols on standard plate readers.

PathHunter™ assays using ProLabel tagged fusion protein:

ProLabel peptide complements with EA very efficiently is referred to has been coined as "high affinity complementation". In one format, EA is specifically localized to the nucleus, while ProLabel fusion proteins maintain the natural cytoplasmic localization of the target protein. Positional Complementation occurs only when ProLabel fusion proteins translocate to the nucleus in response to extracellular stimuli. Assays that measure protein degradation, secretion and membrane trafficking can also be easily configured. Contact us for custom assay development services.

PathHunter™ assays using ProLink tagged fusion protein:

ProLink, a mutant variant of ProLabel sequence, has a lower affinity to EA. For complementation tooccur, the two tagged proteins (EA and ProLink tagged proteins) have to interact due to biological response to a stimulus. This
leads to complementation and formation of active β-galactosidase. Assays that measure receptor activation and cytoplasmic interactions can easily be configured using this principle. Over 200 GPCRs, Receptor Tyrosine Kinases and Nuclear Hormone Receptor assays are available. Contact us for custom assay development services.