NF-κB Signaling Pathway

Nuclear factor-κB (NF-κB) belongs to a family of transcription factors that play pivotal roles in inflammatory responses and immunological reactions. NFκB pathway can be activated by a variety of stimuli, including TNF-α (tumor necrosis factor α), interleukin 1 (IL-1), T and B cell mitogens, bacterial lipopolysaccharide (LPS) and viral proteins. DiscoveRx offers a simple one-step IκB degradation and NFκB translocation assay to study NFκB pathway. These fully optimized, target-validated cell lines provide you convenience, relevant hits and guaranteed performance for screening, profiling or research applications.

Features and Benefits

Short compound incubation times (30-60 minutes); reduce toxicity & false positives
Direct, non-reporter gene, functional Assay
Monitor degradation of IκB
Whole cell, HTS friendly chemiluminescent assay
Transcription independent assay
Increased specificity
Available in multiple backgrounds

Applications

Screen inhibitors for: TNFα signaling, IκB kinases

Assay Principle

DiscoveRx offers PathHunter® IkB functional assays in which IkB (Inhibitor of κB)is tagged with small ProLabel peptide. The assay measures human IκB degradation in response to activation of the NFkB pathway. In the presence of TNFα or other compounds, cells signal through downstream IκB proteins. IκB protein is degraded in response to compound stimulation. The PathHunter® IκB Functional Assay is used with the PathHunter® PK/PL Detection Kit (93-0180).

PathHunter® IκB Degradation Assay

View Categories: Pathway Assays