Targets / Nuclear Receptors

Nuclear Hormone Receptors

Robust, Sensitive Assays with Full-Length NHR

Nuclear Hormone Receptors (NHRs) are ligand-activated transcription factors that, in concert with additional factors, regulate gene expression or repression and play a critical role in endocrine signaling. For discovery of novel compounds targeting NHRs, DiscoveRx offers 26 cell-based assays with full-length NHR, which are ideal for measuring NHR transcriptional activation, translocation, and NHR-coactivator/corepressor interaction. Because our unique NHR assays do not require downstream transcriptional activation or lengthy 16-24 hour compound incubations characteristic of standard reporter gene assays, DiscoveRx’s rapid protocols minimize cell toxicity and false positive rates, yielding results with high specificity.
 


AR ERRα ERα FXR GR
LXRα LXRβ MR PPARα PPARγ
PPARδ PRα PRβ RARα RARβ
RORγ RXRα RXRγ THRα THRβ
Therapeutic Areas

Cell Lines

DiscoveRx offers a comprehensive panel of stable, pre-validated cell lines with full-length NHR to measure nuclear translocation and protein interaction. 

Learn about the Nuclear Translocation assay principle >
Learn about the Protein Interaction assay principle >

Assay Ready Kits

PathHunter® eXpress NHR Assays are an extensive panel of complete, ready-to-use kits that include all cells and reagents to measure NHR translocation and NHR coactivator interaction in whole cells containing full-length NHRs. Free yourself from lengthy cell culture, expensive instrumentation, and complex assay development!

Learn about our assay ready kits >

Biochemical Assays

Quantitatively detect estrogen, progesterone analogs, and cortisol using our suite of validated HitHunter® Assays. Our complete, ready-to-use, biochemical kits include all reagents for competitive assay formats involving chemiluminescent and fluorescent readouts.

Learn more about the HitHunter® assay principle >
See how the HitHunter® Estrogen and Progesterone assay works >
Learn about the Hithunter® Cortisol assay principle >

Cell Culture Kits, Reagents & Consumables

Performance you can trust! Get the highest quality data from your cell-based NHR assays in combination with our lab-certified PathHunter® cell culture media, tissue culture reagents, and plates.

Ligands and Inhibitors

Designed to target a specific nuclear hormone receptor, our purified reference ligands and inhibitors are a convenient and cost-effective way to validate any stable NHR cell line or assay-ready kit.

Detection Reagents

Get optimal assay performance using certified chemiluminescent or fluorescent PathHunter® Detection Reagents that have been validated for use in both 96-well and 384-well microplate formats.

Instruments

Get reliable and reproducible results using our 96-well microplate reader that is ideal for all DiscoveRx chemiluminescent assays.