Wnt-Frizzled Pathway

Wnt signaling plays a pivotal role in development and is implicated in a variety of disease states ranging from cancer, stem cell loss to kidney and reproductive tract defects. Wnt ligands are associated with both β-catenin dependent (canonical) pathway and β-catenin independent pathway, of which the canonical pathway has been extensively researched. DiscoveRx offers a novel cell-based, chemilimunescent assay that measures effects of compounds on any of the upstream targets that effect
β-catenin translocation.

PathHunter^® β-catenin Functional Assay

The PathHunter^® assay monitors the interaction of two proteins in a whole cell, homogeneous assay format using enzyme fragment complementation. The cells have been engineered to express two complementing fragments of β-Gal within different cellular compartments. Read more

Product Features

 Simple pipette-and-read protocol
 Multiple formats
      » One-step, HTS-ready cell line
      » Convenient Off-the shelf kit
 Broad applicability
 Simple chemiluminescent detection

Applications

 Study Frizzled receptor activation or GSK 3β inhibition
 Detect small molecules or functional antibodies
 Use as a biosensor cell line for siRNA application

Available Literature

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