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ProLabel™ and ProLink™ Vectors
Build Protein Trafficking Assays with our Cloning and Expression Vectors
Choose a ProLabel Cloning Vector: ProLink Cloning Vectors are designed such
that the gene of interest is cloned in frame with the complementing fragment of
β-gal termed ProLabel (PL). ProLabel is a high affinity tag used for construction of
assays to directly measure the effect of test compounds on protein translocation,
secretion or degradation in whole mammalian cells. ProLabel Vectors can also be used
to monitor general protein expression in a convenient antibody-free format with our
EAstern Blot Assay Kit. ProLabel Cloning Vectors are engineered for cloning a
selected gene fused to either the N-or C-terminus of ProLabel. The vectors are
available in 3 different reading frames (N1, N2, N3 and C1, C2, C3) to facilitate
preparation of the fusion protein construct.
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Choose a ProLink Cloning Vector: ProLink Cloning Vectors are designed such that
the gene of interest is cloned in frame with the weakly complementing fragment of
β-gal termed ProLink (PK). ProLink is a low affinity tag (~30-fold lower affinity for the
Enzyme Acceptor (EA) than the ProLabel) used for construction of protein-protein
interaction assays, such as NHR:SRCP. The ProLink2 (PK2) tag has higher affinity
for EA than the original ProLink (PK) tag (~3-fold greater) and is useful for weak
protein:protein interactions. For the construction of GPCR cell lines, we have
specialized vectors which can improve the S:B ratio. G-protein Receptor Kinase (GRK)
phosphorylates GPCRs; facilitating recruitment of arrestins. The Arrestin Recruitment
Modifying Sequence (ARMS) is a GRK consensus phosphorylation site deduced from
analysis of GPCRs which generate a high signal:background in the PathHunter assay.
The addition of ARMS can improve assays which have a low S:B ratio. All ProLink
Cloning vectors utilize a minimal CMV promoter for effective expression in mammalian cells.
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Choose a ProLabel or ProLink Expression Vector: ProLabel and ProLink Expression Vectors are available with the 4 kDa tag on a wide variety of proteins.
Product Features
Small, 4 kDa ProLink tag is less likely to perturb normal protein function
Develop novel assays to identify the effects of siRNA, therapeutic antibodies,
molecules, etc.
For instrument compatibility, please click here.
Click on figure to enlarge
Figure 1. Map of the pCMV-PL-C1 Cloning Vector.
Click on figure to enlarge
Figure 2. Map of the pCMV-PK Cloning Vector.
Cloning Vectors
Expression Vectors
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