CREB regulated transcription coactivator (CRTC or TORC) Pathway

cAMP-regulated transcriptional co-activators TORC1 (CRTC1) and TORC2 (CRTC2) are known to regulate the cyclic-AMP-responsive element-binding (CREB) protein. Rising intracellular cAMP levels lead to de-phosphorylation of CRTC1 and CRTC2, which leads to the translocation of CRTC to the nucleus. CRTC translocation is known to be regulated by AMPK (5'-AMP activated kinase), PKA and SIK. TORC2 and AMPK are potential therapeutic targets for Type II diabetes. DiscoveRx offers novel direct, functional cellular assays for TORC1 (CRTC1) and TORC2 (CRTC2) translocation. No more assay development required; a simple mix-and-read microtiter plate-based assay ready-to-use biosensor cell lines is now available!

Product Features

• Simple pipette-and-read protocols
• HTS-ready cell line
• Measures nuclear translocation of CRTC1 and CRTC2
• Simple chemiluminescent detection

Applications

• Measure effect of compounds on CRTC1 & CRTC2 translocation
• Screen or profile compounds against AMPK, SIK or CRTC
• Suitable as a biosensor cell line for siRNA

How does the assay work?

Click here to view assay principle
 

Interested in other diabetes related targets?

Click here to view DiscoveRx assays for diabetes.

New! Custom bulk cell options are available with the purchase of any (PathHunter® Pathway Assays) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.

Don't see your target?

Click here to explore our Custom Assay Development possibilities.

 

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