Sterol regulatory element-binding protein (SREBP) Pathway

Membrane-bound transcription factors, sterol regulatory element binding protein-1 and -2 (SREBP-1 and SREBP-2) mediate homeostasis of cholesterol and fatty acids. SREBP-2 monitors cellular cholesterol levels and responds to low levels of cholesterol by the transcription of genes for HMG-CoA Reductase and other enzymes involved in cholesterol synthesis pathway. Recent studies implicate SREBP-2 in hypercholesterolemia, coronary heart disease, obesity, autophagy and Alzheimer's disease. DiscoveRx offers novel direct, functional cellular assays for SREBP2 translocation. No more assay development required; a simple mix-and-read microtiter plate-based assay is now available!

Product Features

• Simple pipette-and-read protocols
• HTS-ready cell line
• Measures nuclear translocation of SREBP2
• Simple chemiluminescent detection

Applications

• Measure effect of compounds on SREBP2 translocation
• Screen or profile for SITE1 inhibitors
• Functional assay for cholesterol sensing pathway

How does the assay work?

Click here to view assay principle

Interested in other protease assays?

Click here to view DiscoveRx assays for proteases.

New! Custom bulk cell options are available with the purchase of any (PathHunter® Pathway Assays) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.

Don't see your target?

Click here to explore our Custom Assay Development possibilities.

 

Copyright ©2012, DiscoveRx Corporation. TERMS AND CONDITIONS