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Pathway Assays

– ProLabel™ Vectors

ProLabel™ Vectors

Build Protein Trafficking Assays with our ProLabel™ Cloning and Expression Vectors

ProLink Cloning Vectors are designed such that the gene of interest is cloned in frame with the complementing fragment of β-gal termed ProLabel (PL). ProLabel
is a high affinity tag used for construction of assays to directly measure the effect of test compounds on protein translocation, secretion or degradation in whole mammalian cells. ProLabel Vectors can also be used to monitor general protein expression in a convenient antibody-free format with our ProLabel Detection Kit (plate-based) or EAstern Blot Assay Kit (membrane detection). ProLabel Cloning Vectors are engineered for cloning a selected gene fused to either the N-or C-terminus of ProLabel. The vectors are available in 3 different reading frames (N1,
N2, N3 and C1, C2, C3) to facilitate preparation of the fusion protein construct.

Choose a ProLabel™ or ProLink™ Expression Vector: ProLabel and ProLink Expression Vectors are available with the 4 kDa tag on a wide variety of proteins.

Product Features

• Small, 4 kDa ProLink tag is less likely to perturb normal protein function
• Develop novel assays to identify the effects of siRNA, therapeutic antibodies,
   small molecules, etc.

For instrument compatibility, please click here.


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Figure 1. Maps of the C-terminal pCMV-PL Cloning Vectors.


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Figure 2. Maps of the N-terminal pCMV-PL Cloning Vectors.

Cloning Vectors

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Products
Description
Catalog #
Price
pCMV-ProLabel C-Pack
C-terminal ProLabel Vector (3)
93-0014
pCMV-ProLabel N-Pack
N-terminal ProLabel Vector (3)
93-0013
pCMV-ProLabel N-Pack and pCMV-ProLabel C-Pack
Includes ProLabel-N Pack and ProLabel-C Pack (N & C)-vectors in all reading frames (6)
93-0023
pCMV-ProLabel-C1
C-terminal ProLabel Vector
93-0010
pCMV-ProLabel-C2
C-terminal ProLabel Vector
93-0011
pCMV-ProLabel-C3
C-terminal ProLabel Vector
93-0012
pCMV-ProLabel-N1
N-terminal ProLabel Vector
93-0007
pCMV-ProLabel-N2
N-terminal ProLabel Vector
93-0008
pCMV-ProLabel-N3
N-terminal ProLabel Vector
93-0009

Expression Vectors

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Products
Description
Catalog #
Price
ProLink™ Expression Vectors
ProLink vectors containing cDNA for GPCR targets
Inquire

Parental Cell Lines

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Products
Description
Catalog #
Price
PathHunter® CHO-K1 EA-Cyto Parental Cell Line
CHO-K1 cells expressing EA restricted to the cytoplasm for developing nucleus to cytoplasm trafficking assays
93-0179
PathHunter® CHO-K1 EA-Nuc Parental Cell Line
CHO-K1 cells expressing EA restricted to the nucleus for developing nuclear translocation assays
93-0004
PathHunter® HEK 293 EA-Nuc Parental Cell Line
HEK 293 cells expressing EA restricted to the nucleus for developing nuclear translocation assays
93-0038
PathHunter® U2OS EA-Nuc Parental Cell Line
U2OS cells expressing EA restricted to the nucleus for developing nuclear translocation assays
93-0178
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