PI3 Pathway

                         
PI3-K/AKT signalling regulates cellular processes such as proliferation, growth, survival, apoptosis, and the upregulation of hypoxia-related proteins. Activation of this pathway involves various receptor tyrosine kinases, such as Insulin or IGFR1 or cytokines to name a few. DiscoveRx offers novel, non-imaging , cell-based and biochemical assays to study various aspects of the PI3 Pathway. Ready to screen, biosensor cell lines allows you to study receptor activation, phosphorylation or downstream foxo translocation in a HTS-friendly cell-based format, while universal, non-antibody ADP accumulation assay provides a screening-friendly in-vitro assay format, for identifying PI3 or AKT inhibitors.

PathHunter® FOXO Translocation assay

The PathHunter assay monitors the interaction of two proteins in a whole cell, homogeneous assay format using enzyme fragment complementation. The cells have been engineered to express two complementing fragments of β-Gal within different cellular compartments. In this system, a small 42 AA enzyme fragment, Read More»

Product Features

 Novel cell-based assay that measures downstream FOXO translocation
 Whole cell, chemiluminescent assay
 Single addition, HTS-friendly format

Applications

 Assay serves as PI3-kinase pathway indicator
 Allows for Screening of inhibitors for PI3-kinase and Akt
 A tool for studying molecules affecting FOXO3 nuclear translocation
  Biochemical assay for PI3/AKT inhibitors

Available Literature

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New! Custom bulk cell options are available with the purchase of any (PathHunter® Cell-Based Kinase) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.

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