NFκB Pathway

Nuclear factor-κB (NF-κB) is a family of highly regulated dimeric transcription factors that play pivotal roles in inflammatory responses and immunological reactions. NFκB pathway can be activated by a variety of stimuli, including TNF-α (tumor necrosis factor α), interleukin 1 (IL-1), T and B cell mitogens, bacterial lipopolysaccharide (LPS) and viral proteins. DiscoveRx offers a simple one-step IκB degradation and NFκB translocation assay to study NFκB pathway. These fully optimized, target-validated cell lines provide you convenience, relevant hits and guaranteed performance for screening, profiling or research applications.

How does the assay work?

DiscoveRx offers a HEK cell line expressing IκB with the ProLabel EFC tag a small peptide epitope (ProLabel) is expressed recombinantly on the intracellular C-terminus of the IκB. In response to TNFα, endogenous TNFa receptor present in HEK cells signal through downstream IκB. Iκb protein is degraded in response to agonist stimulation. The PathHunter® HEK 293 IκB Functional Assay is used with the PL Detection Kit (93-0180) in PathHunter assays for measuring human I-Kappa-B- (IκB, alternate name NFKBIA; NM_020529.2) degradation in response to pathway stimulation.

Product Features

 Novel cell-based assay that measures IκB degradation
 Whole cell, chemiluminescent assay
 Single addition, HTS-friendly format
 Non-reporter gene format with rapid response

Applications

 Reports NFκB activation
 Find NFκB inhibitors
 Monitor TNF signaling

Available Literature

Click here to view what we have available!

New! Custom bulk cell options are available with the purchase of any (PathHunter® Cell-Based Kinase) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.

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