PathHunter^® Mitotic Index Assays

Detection: EFC Chemiluminescent Detection

PathHunter® Mitotic Index Assays are novel biosensor cell assays that uniquely meet the need for easy, cost effective HTS assays to identify compounds or biologicals like siRNAs that inhibit mammalian cell proliferation. PathHunter® Cell Lines and Detection Kits are used together to detect nuclear envelope degradation at mitosis.

The ready-to-use assays advance screening for new cancer therapeutics because, unlike other mitotic assays that require complex and expensive HCA imaging, flow cytometry or antibodies, PathHunter Assays are homogeneous, without antibodies, and results are obtained using standard plate readers. HEK 293 (human embryonic kidney) and CHO-K1 (hamster) double-stable cell lines are currently available.

Product Features

• Enables whole-cell, fuctional assays
• Quickly identifies mitotic inhibitors by signal gain
• Also detects non-mitotic inhibitors with signal loss
• Just two steps-add test compound and detection reagent
• Needs no fixation, imaging, or antibody labeling
• Compatible with automated screening
• Uses any standard plate reader
• Minimizes compound interference

Biosensor Cells: A Breakthrough Enabling HTS

These HEK 293 and CHO-K1 biosensor cells uniquely combine a homogeneous
format with the in vivo mammalian cellular context needed to rapidly identify
quality anti-cancer hits. Furthermore, mitotic inhibition in cultured cells increases chemiluminescence, providing positive signal gain, rather than the negative read-out common in other assays for anti-proliferatives.

Anti-cancer Agent Discovery & Characterization

PathHunter Mitotic Index Assays enable assessment of mitotic activity in cells treated with anti-cancer test compounds or siRNAs. The assays are sensitive enough to differentiate between inhibitors of mitosis and other cell cycle phases, such as DNA replication in S phase. Validation studies also show they positively indentify mitotic inhibitors among known anti-cancer compounds, with low false positives.

Available Literature

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