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PathHunter® EA and ProLabel™ Detection Kits
The PathHunter® EA Detection Kit is used with PathHunter®
Cell Lines that express the Enzyme Acceptor(EA) fragment of
β-galactosidase, such as EA-Nuc, EA-Cyto
or EA-Arrestin Parental Cell Lines. This detection reagent formulation includes
ED reagent, a small ProLabel/ProLink-type peptide that complements with EA during assay incubation.
The PathHunter® ProLabel Detection Kit is for use with cell lines expressing ProLabel (PL) or ProLink (PK) expression or cloning vectors. The kit measures the total amount of ProLabel or ProLink-tagged protein expressed in cells.
NOTE: PathHunter® Cell Lines expressing both a Prolabel or ProLink-tagged protein and EA require the PathHunter® Detection Kit (93-0001).
Product Features
Simple, homogeneous assay for detection of either EA or PL/PK expression in cells
Confirm expression of proteins without the necessity of using an antibody against
for your target protein
For instrument compatibility, please click here.
Click on figure to enlarge
Figure 1. EFC activity of PathHunter® Cell Lines
The PathHunter® HEK 293 EA-Arrestin Parental Cell Line and PathHunter CHO-K1 EA-Nuc Parental
Cell Line were seeded at 5,000 cells per well into a 384-well plate. EFC activity was measured
using the PathHunter® EA Detection kit according to the assay procedure. A 10-fold increase in
signal over background was observed and indicated strong expression of EA in both cell lines. The
EA positive control provided produced a comparable signal when used at a 100-fold dilution.
Click on figure to enlarge
Figure 2. EFC activity of CHO-K1 Cell Line expressing IκBα-ProLabel fusion protein
A CHO-K1 cell line expressing IκBα-PL fusion protein was seeded into a 384-well plate at 5,000
cells per well. EFC activity was measured using the PathHunter ProLabel Detection Kit according to
the assay procedure. A 30-fold increase in signal over background was observed and indicated
good expression of the fusion protein in the cells. The PL provided control peptide generated a
comparable EFC signal at a 50-fold dilution.
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