PathHunter® and PathHunter® Flash Detection Kits

The PathHunter® and PathHunter® Flash Detection Kits provide a simple, homogeneous gain-of-signal assay for measurement of enzyme fragment complementation (EFC) activity in live cells. PathHunter Detection Reagents are designed for use with PathHunter cell Lines expressing both the complementing fragments of β-galactosidase (β-gal). All assays have been validated for use in both 96-well and 384-well microplate formats.

Product Features

• Homogeneous and flexible assay protocol
• Simple, gain-of-signal assay
• Robust, reproducible signal (Z' factors > 0.7)
• Minimal compound interference
• Run on any standard benchtop luminometer

Flash Detection

The PathHunter Flash Detection Kit was designed to measure interacellular EFC activity in less than one minute after addition of detection reagents. As a result, receptor activation can be detected very rapidly with minimal disruption of cell biology. This kit is ideal for instruments with onboard fluid handling and rapid chemiluminescent data acquisition, such as Hamamatsu FDSS6000, LumiLux™ Cellular Screening Platform, FLIPRTETRA, etc.

For instrument compatibility, please click here.

Glow Detection

The PathHunter Detection Kit was designed to measure intracellular EFC activity within 2 hours after addition of detection reagents. This kit is ideal for use with any standard benchtop luminometer.

Kit Selection Guide

PathHunter^® Detection Kits are compatible with a wide range of PathHunter cell lines. For a list of compatible cell lines, click here.

Available Collaterals

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