PathHunter™ CSF3R-JAK1 Functional Assays
Figure 1. PathHunter™ cells expressing were incubated with increasing concentrations of the respective agonist/antagonists. PathHunter Detection Reagents (93-0001) were used in this assay. Assays were run in a 384-well plate and read on a standard luminometer. The assay detects CSF3R activation (G-CSF stimulation),CSF3R- JAK1 interaction and JAK1 inhibitors.
PathHunter™ PRLR-JAK1 Functional Assay
Figure 2. Agonist Dose response: Cytokine receptors lack kinase domain. They signal through the JAK/STAT pathway. PathHunter™ PRLR-JAK1 cells express Prolactin receptor and JAK1 kinase with PLCG1 as the SH2 containing domain. Upon ligand (Prolactin) binding, the PRLR gets phosphorylated. Phosphorylated cytokine receptors recruits the SH2 containing domains and this interaction forces complementation of the B-gal fragment and addition of a chemiluminescent substrate yields a signal that can be detected in any standard luminometer.