Chemokine CCR5 Receptor and Atherosclerosis

Atherosclerosis affects 64 million Americans and is the condition in which an artery wall thickens as the result of a build-up of fatty materials. The proatherogenic chemokine ligand, CCL5, binds to the CCR1 and CCR5 chemokine receptors expressed on various cell types involved in atherosclerotic plaque formation and is therefore considered prognostic for disease progression. Read more from Zernecke et al in their review on Chemokines in Atherosclerosis.

Many chemokines undergo proteolytic processing which can generate cleaved products that are either more active, antagonistic or inactive compared to the native form. While CCL5 can be detected by standard immunoassays (ELISA), an assay that measures chemokine bioactivity direct in plasma samples may be more useful than a standard ELISA.

DiscoveRx offers the CCR5 receptor in both the assay-ready and live cell formats.
–View assay-ready performance using neat heparinized plasma
–View assay-ready vs ELISA performance for a cohort of coronary bypass patients

PathHunter™ Mitotic Index Assays
Biosensor Cells for Cancer Drug Discovery

Cancer occurs as a result of altered regulation of cell cycle leading to unchecked cell proliferation and subsequent tumor formation. Expectedly, most of the cancer therapeutics target cell cycle and cell proliferation events. The PathHunter™ Mitotic Index Assays are cell-based assays specifically designed to identify compounds that affect the various stages of the mammalian cell cycle and are therefore perfectly suited to be used in Cancer Drug Discovery. These cell lines are engineered with nuclear (EA) - and cytoplasmic-localized (PL) fragments of β-galactosidase (β-gal). At mitosis, the nuclear envelope breaks down, allowing the two β-gal fragments (EA and ProLabel™) to complement forming an active enzyme which subsequently hydrolyzes a chemiluminescent substrate to generate signal.

•Offer HEK 293, CHO-K1 and U2OS double-stable cell lines
•Homogeneous (no wash steps), cell-based assays
•Identify compounds affecting various stages of the cell cycle
•Identify siRNAs or biologics that inhibit proliferation in mammalian cells

For additional cancer-related assays offered by DiscoveRx, please visit:
http://www.discoverx.com/charts/cancer_gpcr_chart.php
http://www.discoverx.com/charts/cancer_kinase_chart.php
http://www.discoverx.com/charts/cancer_nhr_protease_chart.php