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Kinases

- The Broadest Panel of Kinase Solutions for Characterization and Screening

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DiscoveRx Kinase Assays

Kinases are a large and complex class of highly druggable target proteins that are inherent to most signaling pathways. DiscoveRx offers the broadest panel of kinase solutions for functional activity, characterization, profiling, screening and pathway information. These generic, no-wash assays include cell-based and in-vitro assays. The cell-based kinase portfolio provides a cellular context to the kinase activation and use full length proteins well-suited for identifying novel inhibitors and antibodies, while the in-vitro assays provide quick and easy screening approach for both active and unactive kinases.

Product Features

Homogeneous, non-radioactive assays
Choice of either target specific cell-based assays or universal
assays for purified kinase
Broad applicability to many target classes
Simple, one to two step protocol - saving you time during assay
development and high throughput screening providing you convenience

Cell-Based

Receptor Tyrosine
Kinase Assays

PathHunter® receptor tyrosine kinase assays are whole cell functional assay, based on the interaction between receptor tyrosine kinases and SH2 binding domain. The assay is ideal for novel inhibitor screening, characterization and antibody therapeutics. Read more»

Targets: TrkA, TrkB, TrkC, INSR, IGFR1, FGFR4, EphB4, PDGFRb, ErbB4, ErbB3/B4, Flt-3, c-Met, c-Ret, DDR1, DDR2, PDGFRα, EGFR, c-KIT, EphB2, EphB3, EphA5, EphA7. Click here to view all available targets.

Cytosolic Tyrosine
Kinase Assays

PathHunter® protein:protein interaction system has been extended to study receptors without kinase domains that couple to cytosolic kinases. Read more»

Targets: JAK1, JAK2, PRLR, GHR, CSF3R

Pathway Assays for
downstream kinases

PathHunter® pathway assays are novel protein-protein interaction assays for pathway analysis. These are cell-based functional assays. Read more»

Targets: AKT1, AKT2, PI3K, GSK-3β, IKK-α, IKK-β, CHEK1, CHEK2, PKA, NPR1, NRF2, SREBP2, TORC1, TORC2, XBP1

Biochemical

Activity-Based
Universal Assays

ADP accumulation assays employ a generic, non-antibody based,
non-radioactive method to detect the amount of ADP produced during phosphorylation. This universal assay works with any kinases, ATPase or UTPase with no need for antibody or modified substrates. Read more»

Targets: Protein Kinases, Lipid Kinases, Non-protein Kinases, ATPases, UTPases, GTPases

Binding-Based
EFC Assays

HitHunter® Kinase binding assays are ideal for inactive/low activity kinases. No substrate or antibody is required and hence it gives an opportunity to find novel kinase inhibitors such as ATP competitive binders and allosteric compounds. Read more»

Targets: P38 MAP Kinases, LCK, PKC, SRC, C-Kit, RTK, Serine
Threonine Kinases

Other

PathHunter®
Detection Reagents

Chemiluminescent detection reagents for use with all PathHunter®
Cell Lines. Kits available for either Glow or Flash luminescence.
Read More»

Don't find your assay?

Talk to our experts, we excel in Custom Assay Development!
Or you may be interested in kinase screening and profiling services