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Kinases

– How Does It Work?

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PathHunter^® Assay Principle (Panel A)
In this PathHunter® assay approach, a small peptide epitope (ProLink) is expressed recombinantly on the intracellular C-terminus of the Receptor Tyrosine Kinase.
SH2 domains is co-expressed with a larger sequence, termed enzyme acceptor (EA). Activation of receptor results in Dimerization and Phosphorylation of RTK with subsequent interaction with SH2 protein. This interaction generates an active
β-galactosidase enzyme (complementation of β-gal fragments PK and EA), which is detected using a chemiluminescent substrate (Panel A). The assays can be run on 96-well, 384-well or 1536 well and read on standard plate luminometers.

Applications for the PathHunter^® Cell-based Kinase technology
PathHunter® assay measures ligand binding, phosphorylation interaction with adaptor proteins of receptor tyrosine kinases. Use of full length receptor protein allows identification of anti-ligand, anti-receptor/Neutralizing and anti-dimerization antibodies (Panel B). It is ideal for small molecule inhibitors as well (Panel C).