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PathHunter® Receptor Tyrosine Kinase Assays
Receptor Tyrosine Kinases are implicated in disease states such as cancer, metabolic disease, inflammatory disease, cardiovascular and many other disease areas. Biochemical assay uses purified kinase domain, synthetic substrates and lack biological context. A need to understand the kinases in a cellular context prompted DiscoveRx to develop a system to report tyrosine phosphorylation status of proteins in their natural cellular environment. PathHunter® Cell-Based Kinase assay monitors the interaction between wild type, full length receptor proteins with SH2 phosphotyrosine binding domains. The assay does not require antibodies or washes and can be easily adapted to a one-step HTS-friendly assay.
Product Features
Ligand induced functional assay
Whole cell, HTS-friendly, chemiluminescent assay
Uses full length receptor tyrosine kinase
No wash, single addition
For instrument compatibility, please click here.
How does it work?
PathHunter® Receptor Tyrosine Kinase Functional Assay cell line is based on DiscoveRx's PathHunter® technology. In this PathHunter assay approach, a small peptide epitope (ProLink) Learn More»
Applications
Find hits from primary and secondary screens
DiscoveRx small molecule inhibitors
Detect activating and inhibiting antibodies
Investigate cell surface interactions
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Available Literature
Click here to view what we have available!
New! PathHunter® Certified Cell Plating Reagents & Control Ligands
New! Custom bulk cell options are available with the purchase of any (PathHunter® Cell-Based Kinase) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.
Don't see your target?
Click here to explore our Custom Assay Development possibilities.
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