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In this PathHunter® assay approach, a small peptide epitope (ProLink) is expressed
recombinantly on the intracellular C-terminus of the Receptor Tyrosine Kinase (RTK).
SH2 domains is co-expressed with a larger sequence, termed enzyme acceptor (EA).
Activation of receptor results in Dimerization and Phosphorylation of RTK with
subsequent interaction with SH2 protein. This interaction generates an active
β-galactosidase enzyme (complementation of β-gal fragments PK and EA), which is
detected using a chemiluminescent substrate (Panel A).
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