PathHunter^® eXpress Receptor Tyrosine Kinase Functional Assays

PathHunter® eXpress Receptor Tyrosine Kinase Functional Assays provide a highly target-specific whole-cell assay that measures receptor tyrosine kinase activation, phosphorylation and recruitment of SH2 domain proteins. The eXpress kits offer a complete, convenient kit that includes pre-validated cells and reagents to ensure robust and reliable performance every time you run the kit. By eliminating lengthy, time-consuming and expensive cell culture, PathHunter eXpress kits enable you to identify functional antibody or small molecule inhibitors against your target receptor tyrosine kinase of interest.

Product Features

 No tissue-culture required
 Target-specific functional assay for receptor tyrosine kinases
 Optimized protocol that eliminates assay development
 Assay-ready frozen cells in a 96-well format.
 Read on any standard benchtop luminometer

How does it work?

PathHunter® eXpress Receptor Tyrosine Kinase Functional Assay kits come with pre-validated ready-to-assay cells, detection reagents, cell plating media and plates. Simply thaw and plate the cells, and run the assay with your compounds. Assay-ready frozen cells are based on DiscoverRx's PathHunter® technology, Learn More»

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Figure 1. Agonist and Small molecule inhibitor Response
PathHunter® PDGFRb (Panel A) TrkC (Panel B) Cells were plated in a 96-well plate at 10,000 cells/well and incubated for 24 hours or 48 hours @ 37^oC. Subsequently the cells were stimulated with test compound/s. PDGFRb for 3 hours in panel A and K252a for 1 hr and then with EC90 of NT3 (agonist), for 3 hours. Signal was detected using the PathHunter® Detection Reagents according to the recommended protocol.

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