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DiscoveRx: Kinase - PathHunter™ Cell-Based Kinase Assays

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Kinases

– Whole-cell Assay Platform for Screening Cytosolic Tyrosine Kinase Activity

PathHunter™ Cytosolic Tyrosine Kinases


Unlike receptor tyrosine kinases, the cytokine receptors lack kinase activity and are phosphorylated by cytosolic tyrosine kinases. The interleukin receptors, prolactin, leptin, Erythropoietin (EPO), Thrombopoietin Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) signal through JAK-STAT pathway, where the receptors are phosphorylated by JAK kinases (which are members of the cytosolic tyrosine kinases). Since, these JAK kinases mediate signalling of several of the receptors involved in autoimmune diseases and cancers, these are viewed as promising new targets for drug discovery. DiscoveRx offers a simple screening friendly cell-based assay for cytokine receptors and the cytosolic tyrosine kinases.

How does the assay work?

To detect the phosphorylation status of cytokine receptors the ProLink is fused to the c-terminus of the target receptor, and co-expressed with a phosphotyrosine binding domain fused to the complementing fragment, EA. Upon activation and phosphorylation of the receptor by the cytosolic kinase, the SH2 domain binds to
the phosphorylated residues. Specificity for a specific kinase can be obtained by
co-expression of specific tyrosine kinases.

Product Features

Novel cell-based assay
Whole cell, HTS-friendly, chemiluminescent assay
Simple one-step, HTS-ready format

Applications

Detect Cytokine receptor phosphorylation
Detect agonists or inhibitors for Cytokine receptor pathway activation
Monitor the role of cytosolic receptor kinases such as JAK1, JAK2 or JAK3
 on cytokine receptor phosphorylation
Identify JAK inhibitors

Available Collaterals

click here to view what we have available!

Target
Catalog #
SH2 Domain
   Details
Price
CSF3R-JAK1
93-0564C3
SHC1
PRLR-JAK1
93-0686C3
PLCG2
PRLR-JAK2
93-0687C3
PLCG2

New! Custom bulk cell options are available with the purchase of any (PathHunter™ Cell-Based Kinase) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.

Don't see your target?

Click here to explore our Custom Assay Development possibilities.

 

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