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PathHunter® Cytosolic Tyrosine Kinases
Unlike receptor tyrosine kinases, the cytokine receptors lack kinase activity and are phosphorylated by cytosolic tyrosine kinases. The interleukin receptors, prolactin, leptin, Erythropoietin (EPO), Thrombopoietin Granulocyte-Macrophage Colony-Stimulating Factor (G-CSF) signal through JAK-STAT pathway, where the receptors are phosphorylated by JAK kinases (which are members of the cytosolic tyrosine kinases). Since, these JAK kinases mediate signalling of several of the receptors involved in autoimmune diseases and cancers, these are viewed as promising new targets for drug discovery. DiscoveRx offers a simple screening friendly cell-based assay for cytokine receptors and the cytosolic tyrosine kinases.
How does the assay work?
To detect the phosphorylation status of cytokine receptors the ProLink is fused to
the c-terminus of the target receptor, and co-expressed with a phosphotyrosine
binding domain fused to the complementing fragment, EA. Upon activation and
phosphorylation of the receptor by the cytosolic kinase, the SH2 domain binds to
the phosphorylated residues. Specificity for a specific kinase can be obtained by
co-expression of specific tyrosine kinases.
New! PathHunter® Certified Cell Plating Reagents & Control Ligands
All PathHunter Certified reagents have been designed, validated and optimized for use with each PathHunter® cell-based assay. These reagents provide a convenient solution for minimizing your assay development time and ensuring optimal and reproducible results every time. Read More»
Product Features
Novel cell-based assay
Whole cell, HTS-friendly, chemiluminescent assay
Simple one-step, HTS-ready format
Applications
Detect Cytokine receptor phosphorylation
Detect agonists or inhibitors for Cytokine receptor pathway activation
Monitor the role of cytosolic receptor kinases such as JAK1, JAK2 or JAK3
on cytokine receptor phosphorylation
Identify JAK inhibitors
Available Literature
Click here to view what we have available!
New! Custom bulk cell options are available with the purchase of any (PathHunter® Cell-Based Kinase) clone. Package sizes range from 100 million to 2 billion cells. Frozen, assay-ready bulk cells will expedite your screening campaigns and ensure reproducible results. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Click here for a quote.
Don't see your target?
Click here to explore our Custom Assay Development possibilities.
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