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Kinases

– Universal Assays for Kinase Screening

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PathHunter® Biochemical Kinase Assays Literature

Brochures/Flyers

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The Broadest Panel of Kinase Solutions for Characterization, Profiling & Screening

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Finally, Include Unactive Kinases in Your Next Screening Campaign - HitHunter^® Kinase Binding Assays

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HitHunter^® Kinase Binding Assays

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ADP Hunter™ and ADP Quest™ Assays for Kinases and ATPases - Screen Compound Libraries and Characterize Hits by Measuring ADP Accumulation

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ADP Quest™ and ADP Quest™ HS ADP Accumulation Assay for Kinases and ATPases - Single-Well Real Time Kinase and ATPase Kinetics

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Presentations

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Evaluation of a homogeneous approach for the identification of kinase inhibitors, using β-galactosidase (β-gal)

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Application Notes/White Papers/Booklets

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DiscoveRx ADP Hunter HS Assay Detects PI-Kinase Activity

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ADP Quest™ HS kinetic assay with Mithras LB 940

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ADP Hunter™ (Plus & HS) and ADP Quest™ (HS) Assay Optimization Checklist and Instrumnet Settings

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384, Low Volume 384, & 1536-Well ADP Hunter� HS Assay Protocol & Performance

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Posters

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Optimized kinase screening with DiscoveRx ADP Quest™ and PathHunter® assays on the SpectraMax® Paradigm® platform

A High Sensitivity Assay for Kinetic Analysis of Kinases and ATPases Based on Measurement of ADP Accumulation

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Optimized ADP Accumulation Assays for PI-3 Kinase for Primary Screening, Kinase and Kinase Inhibitor Characterization

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ADP Quest™ and ADP Quest™ HS: Universal AdP Accumulation Assay for Enzyme Kinetics

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ADP Hunter™ Plus: A Rapid, Quantitative HTS Assay for GDP and UDP

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ADP Hunter™ HS and Quest™ HS: Improved ADP accumulation assays for kinase primary screening and inhibitor characterization/lead optimization

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A Technique Using β-galactosidase Enzyme Fragment Complementation to Directly Characterize Inhibitor Binding to Active and Unactive Kinases

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A New High-Throughput Screening Platform for p38-MAP Kinase Inhibitors in a Kinase Binding Mode

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Bibliography

Title

1. Katja Kristan, Miha Kotnik , Marko Oblak , Uro Urleb J Biomol Screen (2009) ,New High-Throughput
Fluorimetric Assay for Discovering Inhibitors of UDP-N-Acetylmuramyl-l-Alanine: d-Glutamate (MurD)
Ligase, 14, (4), 412-418

2. Xudong Huang, Patrick Finerty Jr. John R. Walker, Christine Butler-Cole Masoud Vedadi, Matthieu
SchapiraSirlester A. Parker, Benjamin E. TurkDebra A. Thompson and Sirano Dhe-Paganon, J. Struct Biol .
(2009), Structural insights into the inhibited states of the Mer receptor tyrosine kinase, 165 (2), 88-96

3. Charter NW, Kauffman L, Singh R, Eglen RM., J Biomol Screen (2006), A Generic, Homogenous Method for
Measuring Kinase and Inhibitor Activity via Adenosine 5'-Diphosphate Accumulation, 11(4), 390-399

4. Zhang B, Senator D, Wilson CJ, Ng SC. Anal Biochem (2005), Development of a high-throughput robotic
fluorescence-based assay for HsEg5 inhibitor screening , 345 (2), 326-335

5. Vainshtein I., Silveria S., Kaul P., Rouhani R., Eglen R and Wang (2002) A High-Throughput, Nonisotopic,
Competitive Binding Assay for Kinases Using Nonselective Inhibitor Probes (ED-NSIPTM) J. Journal of
Biomolecular Screening,, 7(6): 507-514

6. Guido J.R. Zaman, Miranda M.C. van der Lee, Jacobus J. Kok, Rob L.H. Nelissen, Elma E.M.G. Loomans
(2006) Enzyme Fragment Complementation Binding Assay for p38a Mitogen-Activated Protein Kinase to Study the Binding Kinetics of Enzyme Inhibitors ASSAY and Drug Development Technologies. Aug 2006,
Vol. 4, No. 4: 411-420

7. Wesche H., Xiao S-H, and Young S.W. (2005) High Throughput Screening for Protein Kinase Inhibitors.
Combinational chemistry & High Throughput Screening 8:181-195