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ADP Quest™ & ADP Quest™ HS*
Activity Based Universal Assay for Any Kinase or ATPase
ADP Quest™ assay is a unique tool for researchers for profiling enzyme activity of ANY purified kinase or ATPase. The assay employs
a generic, non-antibody based, non-radioactive method to detect the amount of ADP produced during phosphorylation. ADP Quest or the high sensitivity ADP Quest HS is a
useful tool to determine Michaelis-Menten kinetics and to profile inhibitor mode of action and potencies. The assay is for research use only and has been validated in
96- and
384-well formats.
Product Features
• Compatible with any Kinase: Includes low activity enzymes, lipid kinases, histidine
kinases, protein kinase, non-protein kinases, ATPase, UTPase or GTPase
• Kinetic and Endpoint Capability: Perform real time kinase kinetics at a constant
ATP concentration
• Superior tolerance to ATP: characterize enzyme and run at or below Km
• Compatible with peptide substrates and whole proteins
• Homogeneous and non-radioactive assay: No HOT substance NO multiple
additions, Mix and Read and HTS ready
For instrument compatibility, please click here.
For phosphotransferases such as Kinase, ATPase, UTPase and & GTPase validated with these assays, please click here.
Applications: Characterize Your Kinase within an Hour and Save Months of Assay Development Time
• Obtain Km for your substrate
• Profile kinase and compound
• Determine inhibitor mode of action & measure weak inhibitors
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Available Literature
Click here to view what we have available!
Measuring ADP Accumulation
Kinases use ATP to phosphorylate a substrate. For every substrate molecule phosphorylated, an ADP molecule is generated. The ADP Quest & Quest HS measures total ADP by an enzyme coupled reaction. ADP Quest™ produces a positive fluorescent signal that is directly proportional to the ADP concentration. The fluorescent signal is red-shifted, minimizing interference from fluorescent compounds. Unlike the ATP depletion approach to generic kinase studies, ADP Quest gives robust performance even with high ATP (up to 200 µM) or low activity enzymes, and there is no second enzyme competing for ATP. The ADP Quest assay has been successfully implemented in both endpoint and kinetic modes.
*Covered by US Patent #7410755
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