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High Throughput Kinase Screening, ADP Hunter™ PLUS & ADP Hunter™ HS-DiscoveRx

PRODUCTS & SERVICES

Kinases

- Universal Assays for Kinase Screening

ADP Hunter™ PLUS & ADP Hunter™ HS*

Screen Compound Libraries and characterize hits

ADP Hunter™ PLUS and ADP Hunter™ HS are designed for high throughput screening of kinases or ATPases. They are single step assays that are ideally suited for detection of both high and low activity kinases or ATPases. The assays tolerates a broad range of ATP concentrations, and can use either unmodified peptides or whole proteins as substrates.

Product Features

• Applicable to any kinase
• Homogeneous, antibody-free assay
• High DTT tolerance (2 - 3 mM)
• High precision and reproducibility (Z' > 0.75)
• Compatible with a wide range of fluorescent plate readers

Applications

• Screen low activity kinases or ATPases
• End-point read-out for UTPases
• Ideal for 1536 applications


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For phosphotransferases such as Kinase, ATPase, UTPase and & GTPase validated with these assays, please click here.

Available Literature

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For instrument compatibility, please click here.

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Product
Catalog #
Size (mL)
Datasheet
Price
ADP Hunter™ HS - Generic Kinase Assay for HTS
90-0088S
4 (200-400 dp)
ADP Hunter™ HS - Generic Kinase Assay for HTS
90-0088
8 (400-4K dp)
ADP Hunter™ HS - Generic Kinase Assay for HTS
90-0088L
24 (5K-50K dp)
ADP Hunter™ Plus - Generic Kinase Assay for HTS
90-0083
8 (400-4K dp)
ADP Hunter™ Plus - Generic Kinase Assay for HTS
90-0083M
24 (1.2K-12K dp)
ADP Hunter™ Plus - Generic Kinase Assay for HTS
90-0083L
100 (5K-50K dp)
ADP Hunter™ Plus - Generic Kinase Assay for HTS
90-0083XL
500 (10K-250K dp)
90-0099
7 mg
Kinase Assay Buffer A
92-0008A
20 mL
Kinase Assay Buffer B
92-0008B
20 mL
Kinase Assay Buffer C
92-0008C
20 mL
Kinase Assay Buffer D
92-0008D
20 mL

Measuring ADP Accumulation

Kinases use ATP to phosphorylate a substrate. For every substrate molecule phosphorylated, an ADP molecule is generated. The ADP Hunter PLUS & Hunter HS measures total ADP by an enzyme coupled reaction. It produces a postive fluorescent signal that is directly proportional to the ADP concentration. The fluorescent signal is red-shifted, minimizing interference from fluorescent compounds. Unlike the ATP depletion approach to generic kinase studies, ADP Hunter PLUS/HS gives robust performance even with high ATP (up to 200 µM) or low activity enzymes, and there is no second enzyme competing with ATP.


*Covered by US Patent #7410755

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