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PathHunter® eXpress β-Arrestin Ortholog GPCR Assays
PathHunter® eXpress β-Arrestin Ortholog GPCR assays provide a rapid and efficient
method for for species selectivity or SAR/lead optimization of your lead compound.
The high serum tolerance inherent with the PathHunter β-Arrestin technology also
allows sensitive measurements of the bioavailability of compounds in mouse serum or
plasma samples. Determine effective dosing of your lead compound prior to testing in
expensive animal models or screen analytes from mouse serum or plasma samples in
under 3 hours.
Product Features
• More than 30 mouse and rat GPCRs available
• Off-the-shelf complete kit format
• Pre-validated cells and reagents
• Complete step-by-step protocol
• Assay-Ready frozen cells
• Read on any standard benchtop luminometer
Effective Dosing Prior to Animal Studies
Click on figure to enlarge
Figure 1. Cells eXpressing the mouse Chemokine (C-C motif) Receptor 10 (CCR10) receptor
were treated with increasing concentrations of the mouse (red box) and human (blue triangle)
CCL27 ligands. Pharmacology differences were observed between the two compounds.
Species Selectivity of Your Compound
Click on figure to enlarge
Click on figure to enlarge
Click on figure to enlarge
Figure 2. Cells eXpressing the human (A) and mouse (B) Cannabinoid Receptor 1 (CNR1) were treated with increasing concentrations of the CP55940 and WIN55, 212-2 compounds and dose curves were generated.
New! LumiLITE™ Microplate Reader
Introducing the LumiLITE™ Microplate Reader, a cost effective, 96-well bench top luminometer ideal for use with all PathHunter and cAMP Hunter eXpress GPCR Assays. Learn More»
Kit Contents
• Assay-ready, frozen PathHunter® eXpress cells (2 vials each, 1 x 106 cells/vial)
• Optimized Assay Buffer
• PathHunter® Detection Reagents
• 96-well Tissue Culture Treated Plates
Download the user manual 
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