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PathHunter® eXpress β-Arrestin Orphan GPCR Assays are whole cell, functional assays that directly measure GPCR activity by detecting the interaction of β-Arrestin with the activated GPCR. Because Arrestin is known to interact with a vast majority of GPCRs in a G-protein independent manner, PathHunter® β-Arrestin Orphan eXpress assays are an ideal platform to rapidly de-orphanize receptors.
Product Features
• Off-the-shelf complete kits
• Universal β-Arrestin read-out
• Assay ready frozen cells
• Convenient packaging (100dp; 200dp; 1000dp)
• Easy-to-use, optimized protocol
• Simple, chemiluminescent detection with wide instrument compatibility
Product Benefits
PathHunter® eXpress kits lets you measure GPCR signaling in live cells using a fast and simple chemiluminescent readout. The ultra convenient eXpress kits are ready-to-use, complete kits that contain all the necessary reagents to perform a functional whole cell assay including frozen cells, detection reagents, cell plating reagents and plates, and do not require expensive instrumentation to run. Now you can eliminate lengthy, expensive and time consuming cell culture and make functional testing fast and convenient.
Simple, Convenient Protocol
We grow, freeze and validate the cells using an optimized protocol. Simply thaw and plate the cells and run the chemiluminescent assay 24/48 hours later
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Figure 1. Forzen, assay ready cells combined with a simple protocol and chemiluminescent detection allows you to get results faster.
Performance You Can Trust
For known GPCR targets well-characterized ligands can be used to validate the functional
response of the assay. However, more than 100 Class A GPCRs are still classified as Orphans
(oGPCRs), most likely due to a lack of second messenger response. Since no putative ligands
exist for Orphan GPCR Receptors, DiscoveRx's oGPCRs assays undergo extensive 3-step validation
procedure to consistently ensure optimal, out-of-the box performance.
• Molecular Weight Confirmation
• Basal expression as well as GPCR-PK expression
• Cell Viability
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Easily Deorphanize any Orphan GPCR
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Figure 2. Easily deorphanize any orphan GPCR using validated cell lines
(A) Expression of the GPR35 oGPCR receptor was confirmed by EAstern blot, as shown by the
presence of a 34 KDa band (lane 3). (B) Dose response curve for Zaprinast, a "surrogate" ligand
for GPR35 showing a robust response to agonist.
Kit Components
New! Now available in 3 convenient kit sizes
• Frozen assay ready PathHunter® eXpress cells (1, 2 or 10 vials each, 1 x 106
cells/vial)
• Optimized Cell Plating Reagent
• Chemiluminescent PathHunter® Detection Reagents
• 96-well Tissue Culture Treated Plates
• Manual and Cell Line Specific Datasheet
LumiLITE™ Microplate Reader
DiscoveRx offers an ultimate, one-stop solution for chemiluminescent assay read-outs.
This cost effective 96-well bench top luminometer is compatible with all PathHunter®
and cAMP Hunter™ eXpress chemiluminescent GPCR Assays.
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