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PathHunter® eXpress GPCR Internalization Assays are whole cell, functional assays that enable direct and quantitative measurement of internalized GPCRs in live cells but without imaging, antibodies or radioactivity.
Product Features
• Off-the-shelf complete kits
• Direct and quantitative measure
of internalized GPCRs
• Assay ready frozen cells
• Easy-to-use, optimized protocol
• Simple, chemiluminescent detection
Product Benefits
PathHunter® eXpress GPCR Internalization kits let you measure GPCR signaling in live cells using a fast and simple chemiluminescent readout. The ultra convenient express kits are ready-to-use, complete kits that contain all the necessary reagents to perform a functional whole cell assay including frozen cells, detection reagents, media and plates, and do not require expensive instrumentation to run. Now you can eliminate lengthy, expensive and time consuming cell culture to make functional testing fast and convenient.
Technology Principle: How the Assay Works
DiscoveRx offers 2 novel non-imaging, non-antibody chemiluminescent GPCR internalization assays that provide a direct and quantitative measurement of internalized GPCRs in live cells.
PathHunter® Activated GPCR Internalization Assays detect Arrestin-
mediated GPCR internalization. Learn more»
» Download the user manual (Activated) 
PathHunter® Total GPCR Internalization Assays provide a total measure
of endocytosed receptor. Learn more»
» Download the user manual (Total)
Simple, Convenient Protocol
PathHunter® Activated GPCR internalization assays combine a simple, one-step addtion protocol and standard chemiluminescent detection and are ideally suited for 96- or 384-well analysis.
Figure1. Frozen, assay ready cells combined with a simple, one-step addition protocol and chemiluminescent detection allow you to get results faster.
Click on figure to enlarge
• Combine with traditional second messenger assays
to uncover compound-specific differences in receptor
activation and internalization
• Differentiate between slow and fast recycling receptors
• Ideal for hit confirmation or tertiary screens following
a primary screen
Kit Components
PathHunter® eXpress GPCR Internalization Assays are available in 1 x 96-well and
10 x 96-well plate formats
• Frozen, assay-ready PathHunter® GPCR Internalization eXpress cells
• PathHunter® Chemiluminescent Detection Reagents
• Optimized Cell Plating Reagent
• 96-well Tissue Culture Treated Plates
• Product Insert and Cell Line Specific Datasheet
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