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PathHunter® β-Arrestin GPCR assays are whole cell, functional assays that directly measure GPCR activity by detecting the interaction of β-Arrestin with the activated GPCR. Because Arrestin recruitment is independent of G-protein signaling, these assays offer a powerful and universal screening and profiling platform that can be used for virtually any Gi-, Gs, or Gq-coupled receptor.
Product Features
Over 220 naturally coupled PathHunter® β-Arrestin assays available
Engineered clonal cell lines expressing the human GPCR of interest
Optimized pharmacology, specificity and passage stability
Simple step-by-step protocol
Chemiluminescent detection
Technology Principle: How The Assay Works
PathHunter® β-Arrestin assays monitor GPCR activity by detecting the interaction of
β-Arrestin with the activated GPCR using β-galactosidase (β-gal) enzyme fragment complementation (EFC) technology.
Learn more»
Download the user manual 
Discovery of Novel β-Arrestin Biased Ligands
Activation of the CXCR7 receptor (Chemokine (C-X-C motif) Receptor 7) results in strong Arrestin recruitment even in the absence of second messenger signaling allowing you to uncover novel biased ligands.
Learn more
about the implications of β-Arrestin and G-protein biased ligands.
Read how a combined suite of PathHunter®
and HitHunter® technology platform
can be used to uncover
novel biased ligands.
PathHunter® Certified System
The PathHunter® certified system combines engineered clonal cell lines validated
for stable GPCR receptor expression and optimal assay performance using chemiluminescent PathHunter detection reagents.
Each cell line has been characterized for appropriate GPCR pharmacology, specificity and stability in cell
culture. Learn More»
Figure 1. The PathHunter system results in a simple, homogeneous gain-of-signal
assay that can be run in only a few hours on any standard benchtop luminometer.
For instrument compatibility, please click here.
Want to Measure Calcium Flux and Arrestin Recruitment in the Same Well?
Now you can measure Calcium and Arrestin in the same well using one of our naturally Gq-coupled
PathHunter® Arrestin cell lines. To learn more,
download our
20 min webinar.
Click on figure to enlarge
Click here to view a complete list of Gq-coupled PathHunter® Arrestin Cell Line
Available Literature
Click here to view what we have available!
Don't see your target?
Click here to request our Custom Assay Development Service
Don't want to do cell culture?
New! Bulk cell options are available with the purchase of any PathHunter clone. Package sizes range from 100 million to 3 billion cells. Bulk cells are functionally tested for optimal assay performance and are confirmed to be Mycoplasma-free. Combine frozen, assay-ready bulk cells with PathHunter-Approved Cell Plating Reagent and Control Ligand to expedite your screening campaigns and ensure optimal and reproducible results every time. Click here for a quote.
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