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PathHunter® Detection Reagents - Ultrasensitive, Robust & Reliable
The PathHunter® technology is an adaptation of Enzyme Fragment Complementation (EFC) that provides a novel cell-based assay format for detecting protein-protein interactions, protein degradation and protein translocation. It has been successfully applied to a variety of targets and signaling events including tyrosine kinase activity, GPCR responses and internalization, cytokine receptors, and pathway mapping. The PathHunter® Detection Kits from DiscoveRx is used in conjunction with PathHunter Cell Lines and ProLabel™/ProLink™ expression or cloning vectors inside whole cells. They allow you to detect enzyme fragment complementation or just the ProLink or ProLabel epitopes. The detection reagents are highly sensitive, miniaturizable and can be used for a variety of applications detailed below in the kit selectioin guide.
Product Features
Simple one-step addition
Robust & Reproducible detection
Compatible with 96-well, 384-well, 1536 and 3456 formats
Read on any standard benchtop luminometer
How does it work?
Click on figure to enlarge
Figure 1. The PathHunter® Detection Kit consist of a lysis buffer and substrate mixture that is added as a single solution to stimulated cells. After a short incubation, the PathHunter chemiluminescent signal can be detected using any standard luminometric plate reader.
For instrument compatibility, please click here.
Kit Selection Guide
PathHunter® Detection Kits are compatible with a wide range of PathHunter® cell lines. Click here to view more.
Robust, Sensitive PathHunter® Detection Kits:
Click here to view reagent calculator.
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