Bibliography

GPCRs

Using Ligand-Induced Conformational Change to Screen for Compounds Targeting G-Protein-Coupled Receptors. J Biomol Screen OnlineFirst.  doi:10.1177/1087057106298287 (2007) O'Dowd, BR, Alijaniaram, M, Ji, X, Nguyen, T, and Eglen, RM. 

CXCR1 and CXCR2 Receptors Form Constitutive Homo- and Heterodimers Selectively and with Equal Apparent Affinities.  J. Biol. Chem., 280(31):28663-28674, (2005)
Wilson, S, Wilkinson, G, and Milligan, G.

Characterization of Isoprenaline- and Salmeterol-Stimulated Interactions Between β-Andrenoceptors and β-Arrestin 2 Using β-Galactosidase Complementation in C2C12 Cells.  J.Pharm Exp Ther.  2:839-848 (2005) Carter, AA, Hill, SJ.  

 Functional G Protein-Coupled Receptor Assays for Primary and Secondary Screening.  Combinatorial Chemistry & High Throughput Screening.  8:311-318 (2005) Eglen, RM. 

Cell-based Assays For Screening G Protein Coupled Receptors.  BioScience Technology. May 2005.  Kauffman, L, Olson, KJ, Eglen, RM. 

 An Overview of High Throughput Screening at G Coupled Protein Receptors.  Frontiers in Drug Design & Discovery.  1:97-111 (2005) Eglen, RM.

 Identifying orphan G coupled protein receptors in drug discovery.  Drug Discovery Today: Technologies.  1(1):61-68 (2004) Dunlop, J, and Eglen, RM. 

cAMP Detection Methods in HTS: Selecting the Best from the Rest.  Nature Reviews.  2(3):125-135 (2004) Williams, C.  

A 1536 well cAMP assay for Gs and Gi coupled receptors using enzyme fragment complementation.  Assay Drug Devel Tech; 2:39-49. (2004)Weber, M, Ferrer, M, Zheng, W, Inglese, J, Strulovici, B, Kunapuli, P.

A homogeneous enzyme fragment complementation cyclic AMP screen for GPCR agonists. J Biomol Screen.; 7:515-525. (2002) Golla R, Seethala R.

Kinases

Enzyme Fragment Complementation Binding Assay for p38alpha Mitogen-Activated Protein Kinase to Study the Binding Kinetics of Enzyme Inhibitors. Assay Drug Dev Technol .   4(4):411-20 (2006) Zaman, GJ, Lee, MM, Kok, JJ, Nelissen, RL, Loomans, EE.

A Generic, Homogeneous Method for Measuring Kinase and Inhibitor Activity via Adenosine 5'-Diphosphate Accumulation. J Biomol Screening.   0:1087057106286829v1 (2006) Charter, NW, Kauffman, L, Singh, R, Eglen RM.

High Throughput Screening for Protein Kinase Inhibitors. Combinatorial Chemistry & High Throughput Screening.   8:181-195 (2005)  Wesche, H, Xiao, SH, Young, SW.

Assays for membrane tyrosine kinase receptors: methods for high throughput screening and utility for diagnostics. Future Drugs 5(4):561-571 (2005) Minor, LK. 

A High-Throughput, Nonisotopic, Competitive Binding Assay for Kinases Using Nonselective Inhibitor Probes (ED-NSIP™). J Biomol Screening.  6(7):497-504 (2002) Vainshtein, I, Silveria, S, Kaul, P, Rouhani, R, Eglen, RM, Wang, J. 

Development of a high-throughput robotic fluorescence-based assay for HsEg5 inhibitor screening. Anal Biochem 2005; 345:326-335. Zhang, B, Senator, D,Wilson, CJ, Ng, SC.

Pathway Analysis

A Homogeneous Cell-Based Assay to Measure Nuclear Translocation Using β-Galactosidase Enzyme Fragment Complementation. Assay Drug Dev Technol. 2006 Jun;4(3):263-72. Fung, P, Peng, K, Kobel, P, Dotimas, H, Kauffman, L, Olson, K, Eglen, RM.

Drug Discovery: Versatile Tag for Cell-Based Screening. Genetic Engineering News.  Aug 1 2006 (Vol. 26, No. 14) Olson, K, Mathrubutham, M, Wehrman, T, Eglen, RM.

Antibody-free method for protein detection on blots using enzyme fragment complementation. Biotechniques.  40(3):381-3 (2006) Horecka, J, Charter, NW, Bosano, BL, Fung, P, Kobel, P, Peng K, Eglen, RM.

Homogeneous Assays for Cellular Protein Degradation Using β-Galactosidase Complementation: NF-κB/IκB Pathway Signaling.  Assay Drug Devel Tech.  11(1):823-833 (2003) Zhao, X, Vainshtein, I, Gellibolian, R, Shu, Y, Dotimas, H, Wang, XM, Fung, P, Horecka, J, Bosano, BL, Eglen, RM.

Cell Signaling Braches Out.  Nature Methods 3, 223-229 (2006) Dove A.

Proteases

β Galactosidase Enzyme Fragment Complementation as a High-Throughput Screening Protease Technology.  J Biomol Screening.  9(5):398-408 (2004)  Naqvi, T, Lim, A, Rougani, R, Singh, R, Eglen, RM.

Protein Expression

Large-Scale, High Throughput Validation of Short Hairpin RNA Sequences for RNA Interference. J Biomol Screening. February (2006) Lamarcq, LH, Scherer, BJ, Phelan, ML, Kalnine, NN, Ngueyn, YH, Kabakova, T, Chen, X, Tam, M, Chang, C, Berlon, C, Campos-Gonzales, R, Gao, GJ, Golz,S, Vysotski, ES, Farmer, AA.

BD Creator™ and BD In-Fusion™ ProLabel Screening Kits.  Clontechniques. July (2004).  BD Biosciences Clontech

General Reviews

β Galactosidase Enzyme Fragment Complementation as a Novel Technology for High Throughput Screening.  Combinatorial Chemistry & High Throughput Screening.  6:381-387 (2003) Eglen, RM, Singh, R.

Enzyme Fragment Complementation: A Flexible High Throughput Screening Assay Technology.  Assay Drug Devel Tech. 1(1):97-104 (2002) Eglen, RM.